Nanometric depth resolution from multi-focal images in microscopy

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چکیده

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Nanometric depth resolution from multi-focal images in microscopy

We describe a method for tracking the position of small features in three dimensions from images recorded on a standard microscope with an inexpensive attachment between the microscope and the camera. The depth-measurement accuracy of this method is tested experimentally on a wide-field, inverted microscope and is shown to give approximately 8 nm depth resolution, over a specimen depth of appro...

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ژورنال

عنوان ژورنال: Journal of The Royal Society Interface

سال: 2011

ISSN: 1742-5689,1742-5662

DOI: 10.1098/rsif.2010.0508